Center-Driven Project

Project Title: Chemical Tools and Methods for Identification of Targets of Biologically Active Small Molecules

Principal Investigator: Jeffrey Aubé

Co-Principal Investigators: Brian Blagg, Jeffrey Krise, Blake Peterson, Thomas Prisinzano

Specific Aim 1 (Project Leaders: Blagg, Krise, Prisinzano)
Develop methodology for visualizing and identifying intracellular protein targets using fluorescent molecular probes
• Objective 1: Prepare novel fluorescent, cell-permeable fluorophores
• Objective 2: Develop multifunctional tethers and derived probes
• Objective 3: Develop methods for target identification and localization of probes

Specific Aim 2 (Project Leader: Peterson)
Develop yeast three-hybrid (Y3H) systems as tools for the identification of protein targets of small molecules